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DNA polymerase gamma (Pol gamma) catalyzes trans-strand synthesis and appears to have a dedicated function in some cells to bypass stalled replication forks. A "scissors" model is one possible function for Pol gamma in fork repair. In this model, Pol gamma catalyzes in vitro trans-cleavage of a heteroduplex that links two circular DNA molecules (heteroduplex). We have found that purified Pol gamma catalyzes the trans-cleavage of two 5.6-kb plasmids in the presence of a DNA polymerase delta (Pol delta) accessory factor. Cleavage in the presence of Pol delta was enhanced by ATP. Trans-cleavage also occurred on a linear DNA substrate containing a cis-cleavage site. These results imply that Pol gamma may have the ability to cleave heteroduplexes. This finding suggests a novel repair function of Pol gamma in cells.Can name-brand products really provide a great deal of value for dollars? We think so. That's why all Aveda-brand products are fully refundable, including the ones that are guaranteed to make your hair look its best.
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